RIDA®QUICK Verotoxin / O157 Combi (dipsticks)
Product Code N2202
Intended use:
For in vitro diagnostic use. The RIDA®QUICK Verotoxin / O157 Combi test is a immunochromatographic rapid test for the qualitative determination of Verotoxins (syn. Shiga toxins) and of Escherichia coli serotype O157 in enrichment cultures.
General information:
Enterobacteriacea of the species E.coli are a normal part of the
intestinal flora of humans as well as of many farm animals. E. coli can
be viewed as facultative pathogenic through different, often
plasmid-coded pathogenicity factors or pathogenicity factors
transferred through phages.
This includes, in particular, enterohemorrhagic E. coli (EHEC),
which was identified in 1977 and is the primary cause of severe
illnesses such as hemorrhagic colitis (HC) and hemolytic uremic
syndrome (HUS). They are capable of producing two cytotoxins (Verotoxin
1 and 2). A multitude of other serotypes that can cause severe
illnesses besides the O157 prototype have been described since then.
Therefore, every STEC should be considered to be a potential EHEC.
The current recommended approach in the diagnostic testing of
STEC/EHEC is overnight incubation in mTSB broth containing Mitomycin C
as an inductor for the formation of shigatoxins with the subsequent
immunological detection of the shigatoxins as well as the
shigatoxingens and the isolation of the pathogene
from the enrichment broth.
The RIDA®QUICK Verotoxin / O157
Combi rapid assay enables the extremely specific and simultaneous
detection of shigatoxins and the highly virulent Serotype O157 within
20 minutes from the supernatant of the enrichment broth (e.g. RIDA® Anreicherungsbouillon ; Z1003 ; 25 tubes).
- Antigen detection from an enrichment culture using a fast assay
- Unique assay combination for the simultaneous detection of verotoxins and/or E. coli O157
- An innovative technology enables the color-differentiated detection of both
pathogens - Sensitive detection of all humanpathogenic strains of Shiga toxinproducing
organisms - Specific capturing of all E. coli O157 strains (sorbitol-fermenting and
non-sorbitol-fermenting)
Specifications:
Format: | Box with 25 dipsticks |
Incubation time: | The test result can be read off after 15 minutes. |
Cut-off: | Detection limit:
Stx 1: 2 ng/ml Stx 2: 0.5 ng/ml |
Sensitivity: | for Stx 1 / Stx 2: 85.0 %
for O157: 90.9 % |
Specificity: | for Stx 1 / Stx 2: 98.7 %
for O157: 98.5 % |